K. F. Shim and S. I. Ong, from the Department of Zoology, National University of Singapore, published a research document entitled ‘Iron requirement of the guppy (Poecilia reticulata Peters)’ in the Journal of Aquariculture & Aquatic Sciences, Volume 6, Number 2. The publication is available from petsforum.com.
From the abstract:
The iron requirement of the guppy, Poecilia reticulata Peters, was determined by feeding them eight different levels of iron (0.0, 0.010, 0.020, 0.040, 0.080, 0.320, and 0.640 g/kg dry diet) for fifteen weeks. Growth and feed conversion was not significantly affected by the dietary iron. The red blood cell count, hemoglobin content, and hematocrit value was significantly reduced in fish fed an iron-deficient diet. Iron, copper and zinc content of the liver, spleen, kidney, blood, skin and muscle, heart, gills, and ovary was determined. The iron content was significantly lower in fish fed the iron-deficient diet while the copper content was significantly elevated. However, the zinc content in the organs was not affected by the levels of iron in the diets. the level of iron required by the guppy to prevent microcytic, hypochromic anemia was found to be 0.080 g/kg of diet.